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山形大学大学院理工学研究科 システム創成工学科・バイオ化学工学専攻

〒992-8510 山形県米沢市城南4丁目3番16号
0238-26-3237

研究業績Publications

研究論文

投稿論文

  1. Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, and *Makabe K

  Generation of camelid VHH bispecific constructs via in-cell intein-mediated  protein    trans-splicing

        Protein Eng Des Sel., 30, 15-21, 2017

  1. Ryosuke Sasaki, Soichiro Kitazawa, Ryo Kitahara, Hikaru Nakazawa, Yoshikazu Tanaka, Izumi Kumagai, Mitsuo Umetsu, and *Koki Makabe

Zinc ion-binding activity of an anti-ZnO VHH antibody, 4F2

Chem. Lett. 44, 1309-1311, 2015

doi:10.1246/cl.150537

  1. Biancalana M, Makabe K, Yan S and Koide S

Aromatic cluster mutations produce focal modulations of β-sheet structure

Protein Science, 24, 841-849, 2015

  1. Nakagawa A, Moriya K, Arita M, Yamamoto Y, Kitamura K, Ishiguro N, Kanzaki T, Oka T, Makabe K, Kuwajima K, Yohda M.

Dissection of the ATP-dependent conformational change cycle of a group II chaperonin.

J Mol Biol. 426, 447-459, 2014

10.1016/j.jmb.2013.09.034.

  1. Ochi A, Makabe K, Yamagami R, Hirata A, Sakaguchi R, Hou YM, Watanabe K, Nureki O, Kuwajima K, Hori H.

The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and non-substrate tRNA via an induced-fit process.

J Biol Chem. 2013 Aug 30;288(35):25562-74.

10.1074/jbc.M113.485128.

  1. Sekiguchi H, Nakagawa A, Moriya K, Makabe K, Ichiyanagi K, Nozawa S, Sato T, Adachi S, Kuwajima K, Yohda M, Sasaki YC.

ATP dependent rotational motion of group II chaperonin observed by X-ray single molecule tracking.

PLoS One 2013 May 29;8(5):e64176.

10.1371/journal.pone.0064176.

  1. Yagi-Utsumi M, Kunihara T, Nakamura T, Uekusa Y, Makabe K, Kuwajima K, Kato K.

NMR characterization of the interaction of GroEL with amyloid β as a model ligand.

FEBS Lett. 587, 1605-9, 2013.

10.1016/j.febslet.2013.04.007.

  1. Chandak MS, Nakamura T, Makabe K, Takenaka T, Mukaiyama A, Chaudhuri TK, Kato K, Kuwajima K.

The H/D-exchange Kinetics of the Escherichia coli Co-chaperonin GroES Studied by 2D-NMR and DMSO-Quenched Exchange Methods.

J Mol Biol. 425, 2541-60, 2013.

10.1016/j.jmb.2013.04.008.

  1. Nakamura T, Aizawa T, Kariya R, Okada S, Demura M, Kawano K, Makabe K, Kuwajima K.

Molecular mechanisms of the cytotoxicity of human α-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes.

J Biol Chem. 288, 14408-14416, 2013.

10.1074/jbc.M112.437889.

  1. Makabe K (Corresponding author), Nakamura T, Kuwajima K.

Structural insights into the stability perturbations induced by N-terminal variation in human and goat α-lactalbumin.

Protein Eng Des Sel. 26, 165-170, 2013

10.1093/protein/gzs093.

  1. Mukaiyama A, Nakamura T, Makabe K, Maki K, Goto Y, Kuwajima K.

The Molten Globule of β2-Microglobulin Accumulated at pH 4 and Its Role in Protein Folding.

J Mol Biol. 425, 273-291, 2013

10.1016/j.jmb.2012.11.002. 

  1. Mukaiyama A, Nakamura T, Makabe K, Maki K, Goto Y, Kuwajima K.

Native-state Heterogeneity of β2-Microglobulin as Revealed by Kinetic Folding and Real-time NMR Experiments.

J Mol Biol. 425, 257-272, 2013

10.1016/j.jmb.2012.11.004.

  1. Tomoyori K, Nakamura T, Makabe K (Equal contribution with the 1st author), Maki K, Saeki K, Kuwajima K.

Sequential Four-state Folding/unfolding of Goat α-Lactalbumin and Its N-Terminal Variants

Proteins, 80, 2191-206, 2012.

10.1002/prot.24109

  1. Chen J, Yagi H, Sormanni P, Vendruscolo M, Makabe K, Nakamura T, Goto Y, Kuwajima K.

Fibrillogenic propensity of the GroEL apical domain: A Janus-faced minichaperone

FEBS Letters, 586, 1120-1127, 2012.

10.1016/j.febslet.2012.03.019

  1. Chen J, Makabe K, Nakamura T, Inobe T, Kuwajima K.

Dissecting a Bimolecular Process of MgATP2- Binding to the Chaperonin GroEL.

J Mol Biol. 410, 343-356, 2011.

  1. 真壁幸樹

βシートの構造構築機構

生物物理 50, 126-127, 2010 (レビュー)

  1. Biancalana M, Makabe K, Koide S.

Minimalist design of water-soluble cross-beta architecture.

Proc Natl Acad Sci U S A. 107, 3469-3474, 2010

  1. Nakamura T, Makabe K, Tomoyori K, Maki K, Mukaiyama A, Kuwajima K.

Different Folding Pathways Taken by Highly Homologous Proteins, Goat alpha-Lactalbumin and Canine Milk Lysozyme.

J Mol Biol. 396, 1361-1378, 2010.

  1. Ochi A, Makabe K, Kuwajima K, Hori H.

Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes.

J Biol Chem. 285, 9018-9029, 2010.

  1. Huang J, Makabe K, Biancalana M, Koide A, Koide S.

Structural Basis for Exquisite Specificity of Affinity Clamps, Synthetic Binding Proteins Generated through Directed Domain-interface Evolution.

J Mol Biol. 392, 1221-1231, 2009

  1. Biancalana M, Makabe K, Koide A, Koide S.

Molecular Mechanism of Thioflavin-T Binding to the Surface of beta-Rich Peptide Self-Assemblies.

J Mol Biol. 385, 1052-1063, 2009

  1. Makabe K, Koide S.

The promiscuity of beta-strand pairing allows for rational design of beta-sheet face inversion.

J Am Chem Soc. 130, 14370-14371, 2008

  1. Biancalana M, Makabe K (Equal contribution with the 1st author), Koide A, Koide S.

Aromatic cross-strand ladders control the structure and stability of beta-rich peptide self-assembly mimics.

J Mol Biol. 383, 205-213, 2008

  1. Huang J, Koide A, Makabe K, Koide S.

Design of protein function leaps by directed domain interface evolution.

Proc Natl Acad Sci U S A. 105, 6578-6583, 2008

  1. Makabe K, Biancalana M, Yan S, Tereshko V, Gawlak G, Miller-Auer H, Meredith SC, Koide S.

High-resolution structure of a self-assembly-competent form of a hydrophobic peptide captured in a soluble beta-sheet scaffold.

J Mol Biol. 378, 459-467, 2008.

  1. Makabe K, Nakanishi T, Tsumoto K, Tanaka Y, Kondo H, Umetsu M, Sone Y, Asano R, Kumagai I.

Thermodynamic consequences of mutations in Vernier zone residues of a humanized anti-human epidermal growth factor receptor (EGFR) murine antibody, 528.

J Biol Chem. 283, 1156-1166, 2008

  1. Makabe K, Yan S, Tereshko V, Gawlak G, Koide S.

β-Strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of β-strand pairing

J Am Chem Soc. 129, 14661-14669 2007

  1. Onoda T, Rahman M, Nara H, Araki A, Makabe K, Tsumoto K, Kumagai I, Kudo T, Ishii N, Tanaka N, Sugamura K, Hayasaka K, Asao H.

Human CD4+ central and effector memory T cells produce IL-21: effect on cytokine-driven proliferation of CD4+ T cell subsets.

Int. Immunol. 10, 1191-1199, 2007

  1. Akamatsu N, Yamada Y, Hasegawa H, Makabe K, Asano R, Kumagai I, Murata K, Imaizumi Y, Tsukasaki K, Tsuruda K, Sugahara K, Atogami S, Yanagihara K, Kamihira S.

High IL-21 receptor expression and apoptosis induction by IL-21 in follicular lymphoma.

Cancer Lett. 256, 196-206, 2007

  1. Yan S, Gawlak G, Makabe K, Tereshko V, Koide A, and Koide S

Hydrophobic Surface Burial Is the Major Stability Determinant of a Flat, Single-layer beta-Sheet.

J Mol Biol. 368, 230-243, 2007

  1. Makabe K, McElheny D, Tereshko V, Hilyard A, Gawlak G, Yan S, Koide A and Koide S

Atomic structures of peptide self-assembly mimics.

Proc Natl Acad Sci U S A. 103, 17753-17758, 2006

  1. Asano R, Sone Y, Makabe K, Tsumoto K, Hayashi H, Katayose Y, Unno M, Kudo T, Kumagai I.

Humanization of the bispecific epidermal growth factor receptor x CD3 diabody and its efficacy as a potential clinical reagent.

Clin Cancer Res. 13, 4036-4042, 2006

  1. Makabe K, Tereshko V, Gawlak G, Yan S, Koide S.

Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering.

Protein Science 8, 1907-1914, 2006

  1. Makabe K, Asano R, Ito T, Tsumoto K, Kudo T, Kumagai I, Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.

Biochem Biophys Res Commun. 328, 98-105, 2005

  1. Hayashi H, Asano R, Tsumoto K, Katayose Y, Suzuki M, Unno M, Kodama H, Takemura S, Yoshida H, Makabe K, Imai K, Matsuno S, Kumagai I, Kudo T. A highly effective and stable bispecific diabody for cancer immunotherapy: cure of xenografted tumors by bispecific diabody and T-LAK cells.

Cancer Immunol Immunother. 53, 497-509, 2004

  1. Asano R, Kudo T, Nishimura Y, Makabe K, Hayashi H, Suzuki M, Tsumoto K, Kumagai I. Efficient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant antibody fragment.

J Biochem (Tokyo). 132 903-909, 2002

  1. Asano R, Kudo T, Makabe K (Equal contribution with the 1st author), Tsumoto K, Kumagai I.

Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli.

FEBS Lett. 528, 70-76, 2002

総説・解説

   真壁幸樹, ファルマシア トピックス(論文紹介), 「立体構造情報に基づいたアミロイド形成阻害剤の合理的設計」6月号 (2012)

 

    中村敬,真壁幸樹,桑島邦博 「何がタンパク質のフォールディング経路を決めるのか?」,MedicalBio 10月別冊「揺らぎと生体機能」(寺嶋正秀監修),オーム社,pp. 49-54 (2010).

 

        真壁幸樹 「表面残基エントロピー減少法による結晶化を目指した蛋白質への変異導入 ―βシートモデル蛋白質の結晶化を例として」 蛋白質科学会アーカイブ ,2 , e055 (2009)